The role of specific IL-27-producing DC subsets on the magnitude of the CD8 T cell response to subunit vaccines

Abstract The generation of long-lasting, adjuvant-elicited cellular immunity is a significant unmet medical need. Specifically, the use adjuvants to induce clinically relevant CD8 T cells responses would have great utility in chronic infectious diseases and cancer. Our previous data has shown unique requirement for cell intrinsic IL-27 signaling their response adjuvanted subunit vaccination. Indeed, magnitude IL-27p28 production by cDC1s (CD3/B220 −, CD11c +, XCR1 +) actually predicts generated. are also required present vaccine-derived antigens responding CD8+ cells. However, whether needs be produced same presenting antigen, or non-antigen APCs can produce trans, maximal vaccination as yet unknown. Here, we confirm that responses. Furthermore, mice with Il-27p28 deleted only CD11c+ (IL-27p28 fl/flx CD11c-cre) phenocopied defective seen fully deficient IL-27p28, EIB3, receptor. Interestingly, distribution MPECs (CD127hi) SLECs (KLRG1 hi, CD127 lo) these compared WT (C57BL/6) controls did not vary. Numerous myeloid subsets (cDC2, monocytes) well B make IL-27. Utilizing cell-specific cre expression transgenes conjunction floxed IL27p28, characterized requirements adjuvant elicited response.